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Dr. Rodney Vaughn was born and raised in Brookland, Arkansas. In 1983, he graduated from Brookland High School.

He studied agriculture at Arkansas State University and graduated with a bachelor's degree. He graduated from Louisiana State University's veterinary school in 1993 with the Upjohn Award for Clinical Proficiency in Large Animals. He began his career in Collierville, Tennessee, where he worked for a year before returning to Jonesboro to open Vaughn Veterinary Service, a mobile business.

Dr. Vaughn founded Hilltop Veterinary Hospital in Jonesboro in July 1998 and sold it in February 2015.